Benedict’s solution is used to test for simple sugars such as glucose. It is a clear blue solution which is a combination of copper sulfate, sodium citrate, and sodium carbonate. In the presence of simple sugars, the blue solution changes color to green, yellow, and brick-red, depending on the amount of sugar.
Comes packed in HDPE squeeze bottle with PP flip top cap for dispensing by the drop.
Sugar Test – Benedict’s solution
- Mix small amount of each food sample with distilled water to make a test liquid.
- To a test tube, add 40 drops of liquid to be tested.
- If testing more than one liquid, label each test tube with a marker.
- Add 10 drops of Benedict’s solution to each test tube. Carefully heat the test tubes by suspending in a hot water bath at about 40-50 degrees Celsius for five minutes.
- Note any color change. If sugar is present solution will turn green, yellow, or brick-red, depending on sugar concentration.
Shade of Color |
Approx. Concentration
|
Indication |
| Blue | 0 | No reducing sugar |
| Green solution | < 0.5 | Trace reducing sugar |
| Green ppt. | 0.5 – 1 | Trace reducing sugar |
| Yellow ppt. | 1 -1.5 | Low reducing sugar |
| Orange-red ppt. | 1.5 – 2 | Moderate reducing sugar |
| Brick-red ppt. | >2 | High reducing sugar |










